"EDTA is used to estimate Ca and Mg."
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Ethylenediaminetetraacetic acid (EDTA) is a reagent that forms EDTA-metal complexes with alkaline earth metal ions. In alkaline conditions (pH>9), it forms stable complexes with the alkaline earth metal ions Ca2+ and Mg2+. The EDTA reagent can be used to measure the total quantity of dissolved Ca2+ and Mg2+ ions in a water sample. Thus, the total hardness of a water sample can be estimated by titration with a standard solution of EDTA.