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Question

The organism, which is used for gene transfer in higher organism is
  1. Agrobacterium tumefaciens
  2. E.coli
  3. Acetobacter aceti
  4. Bacillus thuringiensis

A
Bacillus thuringiensis
B
Agrobacterium tumefaciens
C
E.coli
D
Acetobacter aceti
Solution
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Agrobacterium tumefaciens is a pathogen of several dicot plants. It is used for the gene transfer in higher organisms. It is able to deliver a piece of DNA known as 'T-DNA' to transform normal plant cells into a tumor and direct these tumor cells to produce the chemicals required by the pathogen. This bacterium invades plants at the site of wound, transforming them and nearby cells to form a tumor called crown gall.

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Q1
Agrobacterium tumefaciens: Gene transfer in plants :: Bacillus thuringiensis: _________

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Q2
The organism, which is used for gene transfer in higher organism is
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Q3
The organism which is used for gene transfer in higher organism is ___________.
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Q4
Match the organism with its use in biotechnology.

(a)

Bacillus thuringiensis

(i)

Host cell for human insulin production

(b)

Thermus aquaticus

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Taq polymerase

(c)

Agrobacterium tumefaciens

(iii)

Insecticidal protein coding gene

(d)

E. coli

(iv)

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Q5
Agrobacterium tumefaciens is most widely used for gene transfer because
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