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Question

Which of the following shows the correct sequence of steps of plant tissue culture?
$S t e r i l i s a t i o n \to H a r d e n i n g \to S e l e c t i o n o f e x p l a n t \to I n o c u l a t i o n \to R e g e n e r a t i o n \to P l a n t l e t t r a n s f e r$
$S e l e c t i o n o f e x p l a n t \to I n o c u l a t i o n \to R e g e n e r a t i o n \to S t e r i l i s a t i o n \to H a r d e n i n g \to P l a n t l e t t r a n s f e r$
$S e l e c t i o n o f e x p l a n t \to S t e r i l i s a t i o n \to I n o c u l a t i o n \to R e g e n e r a t i o n \to H a r d e n i n g \to P l a n t l e t t r a n s f e r$
$H a r d e n i n g \to S t e r i l i s a t i o n \to S e l e c t i o n o f e x p l a n t \to I n o c u l a t i o n \to R e g e n e r a t i o n \to P l a n t l e t t r a n s f e r$

A

S e l e c t i o n o f e x p l a n t \to S t e r i l i s a t i o n \to I n o c u l a t i o n \to R e g e n e r a t i o n \to H a r d e n i n g \to P l a n t l e t t r a n s f e r

B

S e l e c t i o n o f e x p l a n t \to I n o c u l a t i o n \to R e g e n e r a t i o n \to S t e r i l i s a t i o n \to H a r d e n i n g \to P l a n t l e t t r a n s f e r

C

S t e r i l i s a t i o n \to H a r d e n i n g \to S e l e c t i o n o f e x p l a n t \to I n o c u l a t i o n \to R e g e n e r a t i o n \to P l a n t l e t t r a n s f e r

D

H a r d e n i n g \to S t e r i l i s a t i o n \to S e l e c t i o n o f e x p l a n t \to I n o c u l a t i o n \to R e g e n e r a t i o n \to P l a n t l e t t r a n s f e r

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Solution

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The flowchart shows the steps for Plant tissue culture.
With reference to this, the correct option is
'Selection of explant→Sterilisation→Inoculation→
Regeration→Hardening→Plantlet transfer
'

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