Prokaryotes are the primitive and unicellular organism. It has genetic material in dispersed from as they do not have a membrane bounded nucleus and such type of nucleus is called as the nucleoid. It does not have any membrane bound organelle which makes it different from eukaryotes.
Tissue is a group of similar cells.
Organ consists of similar tissues.
Vacuoles are present in plants.
Hence the correct option is D.
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