126. The DNA extraction was done from an E. coli culture, 60 min after its transfer from 15N to 14N medium. What will be the fraction of hybrid DNA in the total extracted DNA?

Open in App

Solution

Verified by Toppr

Was this answer helpful?

Similar Questions

Light DNA (

14_{N}

) transferred to

15_{N}

labelled culture medium for replication. The ratio of the total number of

15_{N}

and

14_{N}

labeled strands of DNA extracted from the culture after three generations is

View Solution

Matthew Meselson and Franklin Stahl grew E. coli(doubling time is

20

min) in medium containing

^{15} N H_{4} C l

for many generations. Then the E. coli was transferred to medium containing

^{14} N H_{4} C l

. After

40

minutes, the cells were harvested and DNA was extracted and subjected to cesium chloride density gradient centrifugation. The proportion of light and hybrid DNA densities will be.

View Solution

In an experiment, E.coli with

^{15}

N DNA were taken. They were transferred to

^{14}

N medium to grow. What percentage of DNA will have

^{15}

^{15}

^{15}

^{14}

N and

^{14}

^{14}

N after 60 minutes?

View Solution

E. coil cells grown on

^{15} N

medium are transferred to

^{14} N

medium and allowed to grow for two generations. DNA extracted from these cells is ultracentrifuged in a cesium chloride density gradient. What density distribution of DNA would you expect in this experiment?

View Solution

E. coli bacteria grew in a nutrient solution containing a heavy, stable isotope of nitrogen,

^{15} N

. It is then transferred to a medium with the more common light isotope of nitrogen. Then DNA from the cells was extracted and subjected to density centrifugation. The strain of E. coli used to divides once every 20 minutes. The results are illustrated below, the heaviest DNA is extracted from the cells at

View Solution