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Question

A 3' ____________ 5' B
C 5' ____________ 3' D

AB and CD represent two strands of a DNA molecule.
When this molecule undergoes replication, forming a replication fork between A and C in the above.
(i) Name the template strands for replication.
(ii) Using which strand as the template, will there be a continuous synthesis of a complementary DNA strand?

Solution
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Explanation:

(i) The template strand for replication will be: A 3' ____________ 5' B , as DNA replication occurs only in 5' to 3' direction.

(ii) Using the AB strand of DNA there will be a continuous synthesis of DNA as DNA is synthesized in 5' to 3' direction only and for that, the template strand needs to be in the 3' to 5' direction.

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