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A person with a normal near point (25 cm) using a compound microscope with objective of focal length 8.0 mm and an eyepiece of focal length 2.5 cm can bring an object placed at 9.0 mm from the objective in sharp focus. What is the separation between the two lenses? Calculate the magnifying power of the microscope.

Solution
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Focal length of the objective lens,fo=8mm=0.8cm

Focal length of the eyepiece, fe=2.5cm

Object distance for the objective lens,μo=9mm=0.9cm

Least distance of distant vision d=25cm

Image distance for the eyepiece,ve=d=25cm

Object distance for the eyepiece =ue

Using the lens formula, we can obtain the value of ue

1ve1ue=1fe

1251ue=12.5

So, ue=2.27cm

We can also obtain the value of the image distance for the objective lens vo using the lens formula.

1vo1uo=1fo

1vo10.9=10.8

vo=7.2 m

Distance between the objective lens and the eye piece is |ue|+vo

so separation is 2.27+7.2=9.47cm

The magnifying power of the microscope is calculated as

m=vo|uo|(1+d/fe)

7.20.9(1+252.5)=88

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