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Question

A set of bacterial clones, each containing a plasmid or phage is called as
  1. Gene pool
  2. Genome
  3. Gene library
  4. Genophore

A
Gene pool
B
Gene library
C
Genome
D
Genophore
Solution
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A genomic library is a collection of the total genomic DNA from a single organism. The DNA is stored in a population of identical vectors, each containing a different insert of DNA. In order to construct a genomic library, the organism's DNA is extracted from cells and then digested with a restriction enzyme to cut the DNA into fragments of a specific size. The fragments are then inserted into the vector using DNA ligase. Next, the vector DNA can be taken up by a host organism - commonly Escherichia coli or yeast - with each cell containing only one vector molecule. Using a host cell to carry the vector, allows for easy amplification and retrieval of specific clones from the library for analysis.

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