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Standard XII
Biology
Electroporation
Question

Biolistics (gene-gun) is suitable for
  1. Transformation of plant cells.
  2. Disarming pathogen vectors.
  3. Constructing recombinant DNA by joining with vectors.
  4. DNA finger printing.

A
Constructing recombinant DNA by joining with vectors.
B
DNA finger printing.
C
Disarming pathogen vectors.
D
Transformation of plant cells.
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Solution
Verified by Toppr

The correct option is A Transformation of plant cells.
Biolistic gun or gene gun method initially developed to transfer the gene in plant cell directly. It is a method involving the transfer of genes to the plants without the use of any vectors (plasmid etc.). Thus, the correct answer is option A.

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Similar Questions
Q1
The given flow chart depicts the steps to transfer a desirable gene of interest into a plant.Isolation of desirable gene using restriction endonucleases and gel electrophoresis.
Regeneration of plants from the transformed cells to get transgenic plants.
Identify the missing steps (A, B and C) with regard to following statements and select the correct option.(i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule(ii) Selection of transformed cells(iii) Transferring the recombinant DNA molecules to the target cells

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Q2
Arrange the given steps involved in recombinant DNA technology in the correct order.

A. Amplification of desired DNA sequence (gene of interest) with PCR modification
B. Ligation of gene of interest with the vector
C. Removal of RNA and proteins to purify DNA
D. Insertion of recombinant DNA into the host
E. Treatment of cell with cell wall digesting enzyme followed by disruption of the cell membrane
F. Digestion of DNA of interest and vector with suitable restriction enzyme
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Q3
The terms ‘microinjection’, ‘biolistics’ and ‘disarmed pathogen vector’ are related to
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Q4
Assertion (A): DNA ligase plays an essential role in recombinant DNA technology.
Reason (R): The linking of antibiotic resistance genes with plasmid vectors became possible by enzyme DNA ligase.
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Q5
For transformation, micro-particles coated with DNA to be bombarded with biolistic gene gun are made up of?
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