Chromatography is a technique of separating molecular components of a mixture by using the principle of partition coefficientdue to which different molecules move at different rates through a stationary phase and get separated. Chromatography is of several types and one of the types is: Ion Exchange Chromatography. In ion exchange chromatography, beads of cellulose and other materials having negative and/ or positive charge are placed in a column. The mixture is poured over the column. As the mixture passes through the column, its constituents separate according to their charges. The technique is used in purification of insulin, plasma fractionation and separation of proteins. Diethylaminoethyl cellulose (DEAE-C) is a positively charged resin used in ion exchange chromatography, used in protein and nucleic acid purification/ separation.