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Question

Electroporation in cloning is
  1. Introduction of genetic material to transgenic animal.
  2. Introduction of genetic material through viruses.
  3. Manipulation of cells by exposing them to a strong electric field.
  4. Injecting DNA into the egg.

A
Injecting DNA into the egg.
B
Manipulation of cells by exposing them to a strong electric field.
C
Introduction of genetic material through viruses.
D
Introduction of genetic material to transgenic animal.
Solution
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Electroporation or electropermeabilization, is a molecular biology technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane, allowing chemicals, drugs, or DNA to be introduced into the cell. In molecular biology, the process of electroporation is often used to transform bacteria, yeast, or plant protoplasts by introducing new coding DNA.

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