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Question

Exon segments are reunited after splicing by
  1. RNA primase
  2. DNA polymerase
  3. RNA polymerase
  4. RNA ligase

A
RNA primase
B
DNA polymerase
C
RNA polymerase
D
RNA ligase
Solution
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RNA primase synthesizes RNA primer which is a stranded segment (complementary to the template) and has a free 3’ hydroxyl group to facilitate the addition of nucleotides by polymerase enzymes. RNA polymerase synthesizes RNA using DNA template strand by adding the ribonucleotides to a primer in 5' to 3' direction via phosphodiester bonds. Splicing is the removal of introns from the primary transcript and ligation of exons to form a continuous sequence specifying a functional polypeptide. RNA ligase enzyme joins two exons together via phosphodiester bond between 3’ hydroxyl at the end of one exon and a 5’ phosphate at the end of another exon. The correct answer is D.

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Q1
Exon segments are reunited after splicing by
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Q2
Which of the following reunites the exon segments after RNA splicing : -
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Q3
Assertion (A): In eukaryotes, both introns and exons are transcribed to form hnRNA.

Reason (R): The exons are removed to make the final transcript by splicing.
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Q5
Assertion (A): In eukaryotes, both introns and exons are transcribed to form hnRNA.
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