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Question

Gene transfer is present in:-
  1. Hybridization
  2. Tissue culture
  3. Biolistics
  4. Vegetative propagation

A
Biolistics
B
Hybridization
C
Vegetative propagation
D
Tissue culture
Solution
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  • Biolistics including particle bombardment is a commonly used method for genetic transformation of plants when either cells/tissues or intracellular organelles are impermeable to foreign DNA. The plant cell wall is usually impermeable to foreign DNA.
  • Utilization of the gene gun method has shown better results against the various barriers that hinder the delivery of foreign DNA inside the organelle/cell/tissue. Gene transfer through a gene gun is part of the biolistic method. During this method DNA or RNA construct adheres to biological inert particles (such as gold or tungsten) to form a DNA/particle complex.
  • The DNA/particle complex is bombarded on a targeted tissue in a vacuum. This bombardment is achieved by accelerating a powerful shot to the targeted tissue. The high density of the DNA/particle complex increases the bombardment speed toward the targeted tissue, which may result in effective transformation.
  • Only inert metal particles can be bombarded through a solution containing DNA surrounding the cell. So, Biolistic (gene gun) is a technique in genetic engineering in which tiny metal pellets coated with DNA are propelled into living cells at high velocities.
So, the correct option is option A.

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