Haploid plant cultures can be obtained by pollen grains by the technique of pollen culture.
Pollen culture is a technique of production of haploid plants by culture of pollen grains obtained from anther of a plant under aseptic condition on artificial media of known composition. The technique was discovered by Guha and Maheshwari. The pollen's were obtained by squeezing the anthers and surface sterilizing is done with hypochlorite. The obtained pollen's were cultured on nutrient media. The pollens develops into a callus which is treated with auxin it promotes rooting and when it is treated with cytokinin it stimulates cell maturation, differentiation and finally, promotes the development of stem.