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Standard X
Biology
Applications of Biotechnology
Question

Identify the steps that are involved in PCR.
(A) Denaturation
(B) Annealing
(C) Extension
(D) Down stream processing
  1. B, D, C
  2. A, D, B
  3. A, B, C
  4. A, C, D

A
A, B, C
B
B, D, C
C
A, D, B
D
A, C, D
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Solution
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  • PCR stands for a polymerase chain reaction. This is a technique used to amplify a given sample of DNA.
  • In this the first step is to denature the DNA template by melting the hydrogen bonds and making it into a single strand at 94 degrees celsius.
  • The second step involves the annealing step that involves joining the primers to the single-stranded DNA.
  • Then this step is followed by the extension or elongation step that involves the extension of the DNA by the Taq polymerase by the addition of the nucleotides.

Hence, option C is correct.

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