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ILI US Peisive rol CUIiservative (d) Replication is not conservative 69. E.coli cells grown on N15 medium are transferred to N" medium and allowed to grow for two more generations (two cycles of DNA replication). DNA extracted from these cells is centrifuged. What density distribution of DNA would you expect in this experiment ? (a) One low-density band and one intermediate density band (b) One high-density and one low density band (C) Only one intermediate-density band (d) Only one low density band bolou Identify

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Q1
E. coil cells grown on 15N medium are transferred to 14N medium and allowed to grow for two generations. DNA extracted from these cells is ultracentrifuged in a cesium chloride density gradient. What density distribution of DNA would you expect in this experiment?
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Q3
Meselson and Stahl grew bacteria in the presence of a heavy isotope of nitrogen N14. The DNA was then transferred to a growth medium containing nucleotides with a lighter isotope of nitrogen N12 which then proceeded to replicate.
The DNA was centrifuged to separate it into weight forming bands. After one round of DNA replication, there was one band in the middle of the centrifuge tube. After two rounds of DNA replication in the lighter nitrogen, two separate bands of DNA could be seen. This demonstrated that replication is semi-conservative.
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Q4
Choose the correct answer from the alternatives given :
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Q5
E. coli bacteria grew in a nutrient solution containing a heavy, stable isotope of nitrogen,15N. It is then transferred to a medium with the more common light isotope of nitrogen. DNA from the cells was extracted and subjected to density centrifugation. The strain of E. coli is used to divides once every 20 minutes. The results are give below.
Which of the following result would describe the DNA bands in the test tubes, If the extraction and centrifugation of DNA is continue at every 20 minutes?
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