In reversed-phase chromatography, the hydrophobic stationary phase is used. Which of these silica-based compounds can be used as a stationary phase?
Cyano bonded silica
Phenyl bonded silica
Octadecyl (C−18) carbon chain bonded silica
All of these
A
Cyano bonded silica
B
Octadecyl (C−18) carbon chain bonded silica
C
All of these
D
Phenyl bonded silica
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Solution
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In reversed phase chromatography, the stationery phase is hydrophobic in nature.
In the above mentioned options, cyano group, phenyl group and C−18 carbon chain all are hydrophobic in nature. Their interaction with water is not at all good. And hence, being hydrophobic, they can be used as a stationery phase in reversed phase chromatography`.
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