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Question

Large increase in absorption of UV- radiation of 260 nm by melted DNA duplex is called
  1. Position effect
  2. Gaidukov phenomenon
  3. DNA compaction
  4. Hyperchromic effect

A
Gaidukov phenomenon
B
Position effect
C
DNA compaction
D
Hyperchromic effect
Solution
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  • The increase in the absorbance of a material is known as hyperchromicity and the effect is known as the hyperchromic effect.
  • The DNA molecule gets denatured by the hyperchromicity.
  • The absorption of UV radiation by DNA molecule increases when the two single DNA strands get separated by heat or by a heat denaturant or by changing the pH level.
  • So, the correct answer is 'Hyperchromic effect'.

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