Name the three important steps involved in PCR process.
Polymerase chain reaction (PCR) is the process in which the amplification of the gene of interest is carried out with two sets of primers and a thermostable DNA polymerase enzyme Taq polymerase. The process involves the following steps:
- Denaturation: The double-stranded DNA is heated up to 98ooC which causes the hydrogen bonds to break and the two strands get separated.
- Annealing: The two sets of primers are added which bind to the appropriate complementary segment of DNA strand.
- Extension: The Taq polymerase enzyme polymerizes the nucleotide chain using the nucleotides provided in the medium and by using the template strand.