Name the three important steps involved in PCR process.

Question

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Answer 1

Polymerase chain reaction (PCR) is the process in which the amplification of the gene of interest is carried out with two sets of primers and a thermostable DNA polymerase enzyme Taq polymerase. The process involves the following steps:
Denaturation: The double-stranded DNA is heated up to 98 $^{o}$ C which causes the hydrogen bonds to break and the two strands get separated.

Annealing: The two sets of primers are added which bind to the appropriate complementary segment of DNA strand.

Extension: The Taq polymerase enzyme polymerizes the nucleotide chain using the nucleotides provided in the medium and by using the template strand.

Answer 2

Polymerase chain reaction (PCR) is the process in which the amplification of the gene of interest is carried out with two sets of primers and a thermostable DNA polymerase enzyme Taq polymerase. The process involves the following steps:

Denaturation: The double-stranded DNA is heated up to 98 $^{o}$ C which causes the hydrogen bonds to break and the two strands get separated.
Annealing: The two sets of primers are added which bind to the appropriate complementary segment of DNA strand.
Extension: The Taq polymerase enzyme polymerizes the nucleotide chain using the nucleotides provided in the medium and by using the template strand.

Name the three important steps involved in PCR process.

Explain the three steps involved in each cycle of polymerase chain reaction.

Mention the three steps involved in each cycle of Polymerase-chain Reaction (PCR). How is repeated amplification of DNA made possible during PCR

Taq Polymerase is involved in PCR technique

This enzyme remain active during the high temperature including denaturation of double stranded DNA.

Taq Polymerase is involved in PCR technique

Identify the steps that are involved in PCR.
(A) Denaturation
(B) Annealing
(C) Extension
(D) Down stream processing

In the three steps (a, b, c) of polymerase chain reaction, select the correct step

Revise with Concepts

Obtaining the Foreign Gene Product

Isolation of the DNA

Insertion of Recombinant DNA into the Host Cell/Organism

PCR

Cutting of DNA at Specific Locations

Name the three important steps involved in PCR process.

Explain the three steps involved in each cycle of polymerase chain reaction.

Mention the three steps involved in each cycle of Polymerase-chain Reaction (PCR). How is repeated amplification of DNA made possible during PCR

Taq Polymerase is involved in PCR technique

This enzyme remain active during the high temperature including denaturation of double stranded DNA.

Taq Polymerase is involved in PCR technique

Identify the steps that are involved in PCR.(A) Denaturation (B) Annealing(C) Extension (D) Down stream processing

In the three steps (a, b, c) of polymerase chain reaction, select the correct step

Revise with Concepts

Obtaining the Foreign Gene Product

Isolation of the DNA

Insertion of Recombinant DNA into the Host Cell/Organism

PCR

Cutting of DNA at Specific Locations

Identify the steps that are involved in PCR.
(A) Denaturation
(B) Annealing
(C) Extension
(D) Down stream processing