Ovum acquires polarity as two poles become distinct. At one end the cytoplasm is more active and contains the nucleus while at the other, called the vegetal pole, the cytoplasm is less active and contains most of the yolk.
Since nucleus is present at animal pole, this is where sperm penetrates ovum and sperm's nucleus fuses with the nucleus of the ovum to form a zygote.
So, the correct answer is 'Animal pole'.
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