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Question

Pollen culture is used to produce
  1. Hybrids
  2. Haploid plant
  3. Disease resistant plant
  4. None of the above

A
Hybrids
B
Haploid plant
C
Disease resistant plant
D
None of the above
Solution
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Guha and Maheshwari (1964) cultured mature anthers of Datura innoxia with a aim to study meiosis, but accidentally made a more significant observation. They noticed that on nutrient medium containing kinetin, coconut milk or grape juice numerous embryos appeared from the inside of anthers which eventually developed into plantlets. In 1966, these workers confirmed the origin of the embryos from pollen grains. As expected the plantlets of pollen origin were haploids. This report aroused a worldwide interest to develop the technique of anther and pollen culture for producing androgenic haploids of a wide variety of crop plants because of their potential significance in basic and applied genetics and plant breeding.

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