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Question

Polyethylene glycol method is used for
  1. Energy production from sewage
  2. Biodiesel production
  3. Gene transfer without a vector
  4. Seedless fruit production

A
Seedless fruit production
B
Energy production from sewage
C
Gene transfer without a vector
D
Biodiesel production
Solution
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Sewage serves as source of biomass energy by anaerobic digestion to produce biogas. The sewage sludge is exposed to pyrolysis to produce more biogas. Biodiesel is produced from straight vegetable oil, animal oil/fats and waste cooking oil by transesterification of a triglyceride (fat/oil) with an alcohol to form esters and glycerol. Development of fruits without fertilization is known as parthenocarpy; the fruits are seedless with longer shelf life and greater consumer appeal. Polyethylene glycol is used in chemical mediated gene transfer wherein it induces DNA uptake into plant protoplasts
So, the correct answer is 'Gene transfer without a vector'

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