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Standard XII
Biology
What is PCR
Question

Primers are

  1. Chemically synthesised oligonucleotides that are complementary to the regions of DNA
  2. Chemically synthesised oligonucleotides that are not complementary to the regions of DNA
  3. Chemically synthesised, autonomously replicating circular DNA molecules
  4. Specific sequences present on recombinant DNA

A
Chemically synthesised oligonucleotides that are complementary to the regions of DNA
B
Chemically synthesised oligonucleotides that are not complementary to the regions of DNA
C
Chemically synthesised, autonomously replicating circular DNA molecules
D
Specific sequences present on recombinant DNA
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Solution
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Primers are small, chemically synthesised oligonucleotides that are complementary to the sequences, present at 3' end of the template DNA. They hybridise to the target DNA region, one to each strand of the double helix. These primers are oriented with their ends facing each other allowing synthesis of the DNA towards one another.
So, the correct answer is 'Chemically synthesised oligonucleotides that are complementary to the regions of DNA'.

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Similar Questions
Q1
Small chemically synthesised oligonucleotides that are complementary to the regions of DNA are called primers and present at the
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Q2
Primers (forward and reverse) which are synthetic oligonucleotides of 17-30 nucleotides. They are complementary to the sequence present on the desired DNA segment.------Why?
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Q3
Rearrange the following in the current sequences to accomplish an important biotechnological reaction.
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
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Q4
Rearrange the following in the correct sequence to accomplish an important biotechnological reaction: [3]
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
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Q5
Read the following statements carefully and select the correct option:
(A) Source of the restriction enzyme Hind III is E.coli
(B) In biolistic method of gene transfer, mircoparticles made up of gold or tungsten are coated with foreign DNA
(C) Micro-injection method for injecting recombinant DNA is used for animal cell
(D) Primers are chemically synthesized oligonucleotides that are complementary to the regions of DNA in PCR.
How many of the above statement are correct?
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