0
You visited us 0 times! Enjoying our articles? Unlock Full Access!
Question

Assertion :Restriction enzymes cut the strand of DNA to produce sticky ends. Reason: Stickiness of the ends facilities the action of the enzyme DNA polymerase.
  1. Both Assertion and Reason are correct and Reason is the correct explanation for Assertion.
  2. Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion.
  3. Assertion is correct but Reason is incorrect.
  4. Both Assertion and Reason are incorrect.

A
Both Assertion and Reason are correct but Reason is not the correct explanation for Assertion.
B
Assertion is correct but Reason is incorrect.
C
Both Assertion and Reason are correct and Reason is the correct explanation for Assertion.
D
Both Assertion and Reason are incorrect.
Solution
Verified by Toppr

Restriction enzyme, a type of endonuclease, functions by "inspecting" the length of a DNA sequence. Once it finds a recognition sequence, it binds and cut each of the two strands of the double helix at specific point leaving single stranded portions at the ends. This results in overhanging stretches called sticky ends. These are named so because they form hydrogen bonds with their complementary counter parts i.e., they can join similar complementary ends of DNA fragment from some other source with the help of DNA ligase. This stickiness of the ends facilitates the action of the enzyme DNA ligase, not DNA polymerase.

Was this answer helpful?
3
Similar Questions
Q1
Assertion :Restriction enzymes cut the strand of DNA to produce sticky ends. Reason: Stickiness of the ends facilities the action of the enzyme DNA polymerase.
View Solution
Q2
Assertion: The compressibility factor for H2​ and He is (1+PbRT)
Reason: The compressibility factor for H2​ and He can be derived from Van der Waal's equation.
View Solution
Q3

Assertion (A) Order of the reaction can be zero or fractional.

Reason (R) We cannot determine order from balanced chemical equation.

(a) Both assertion and reason are correct and the reason is correct explanation of assertion
(b) Both assertion and reason are correct, but reason does not explain assertion.
(c) Assertion is correct, but reason is incorrect
(d) Both assertion and reason are incorrect
(e) Assertion is incorrect but reason is correct.

View Solution
Q4

ASSERTION: n-Pentane and n-Hexane belong to a homologous series.

REASON: n-Pentane and n-Hexane belong to the same class of hydrocarbons and they differ by a - CH2 - group.


View Solution
Q5

Assertion (A) Order and molecularity are same.

Reason (R) Order is determined experimentally and molecularity is the sum of the stoichimetric coeffcient of rate determining elementary step.

(a) Both assertion and reason are correct and the reason is correct explanation of assertion
(b) Both assertion and reason are correct, but reason does not explain assertion.
(c) Assertion is correct, but reason is incorrect
(d) Both assertion and reason are incorrect
(e) Assertion is incorrect but reason is correct.

View Solution