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Question

Select the wrong statement

  1. Retroviruses in animals have the ability to transform normal cells into cancerous cellsIn microinjection, cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA
  2. Presence of chromogenic substrate gives blue colour colonies if the plasmid in the bacteria does not have an insert
  3. Since DNA is a hydrophilic molecule it cannot pass through cell membranes
  4. DNA is a negatively charged molecule

A
Retroviruses in animals have the ability to transform normal cells into cancerous cellsIn microinjection, cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA
B
DNA is a negatively charged molecule
C
Presence of chromogenic substrate gives blue colour colonies if the plasmid in the bacteria does not have an insert
D
Since DNA is a hydrophilic molecule it cannot pass through cell membranes
Solution
Verified by Toppr

In a micro-injection, recombinant DNA is directly injected into the nucleus of an animal cell.
In another method, suitable for plants, cells are bombarded with high-velocity micro-particles of gold or tungsten coated with DNA. This method is known as biolistics or gene gun.
So, the correct option is ' Retroviruses in animals have the ability to transform normal cells into cancerous cells. In microinjection, cells are bombarded with high velocity microparticles of gold or tungsten coated with DNA'.

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During insertional inactivation, the presence of a chromogenic substrate gives blue coloured colonies if the plasmid in the bacteria does not have an insert. The blue colour is produced by the enzyme

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Q3
Match the column: –
Column I Column II
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(iv) Particle Bombardments (s) Directly injected to the nucleus of animal cell through micro

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The ability to transform a normal cell into a cancerous cell is found in
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Q5
Assertion: Gold or tungsten microparticles coated with DNA are used in biolistics.
Reason: Gold and Tungsten are inert.

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