Insertional inactivation of alphagalactosidase in non-recombinant bacteria
C
Insertional inactivation of alphagalactosidase in recombinant bacteria
D
Inactivation of glycosidase enzyme in recombinant bacteria
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The correct option is C Insertional inactivation of alphagalactosidase in recombinant bacteria Insertional inactivation means inactivation of an enzyme by the presence of an insert (recombinant DNA). In the presence of a chromogenic substrate, active alpha-galactosidase enzyme yields blue coloured colonies (non recombinants). While insertional inactivation of alpha-galactosidase enzyme inactivates the enzyme resulting in white colour colonies (recombinants). Thus, it is due to insertional inactivation of alpha-galactosidase in recombinant bacteria that the colonies of recombinant bacteria appear white in contrast to the blue colonies of non-recombinant bacteria.
So, the correct answer is 'Insertional inactivation of alphagalactosidase in recombinant bacteria'.
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Q1
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because
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Q3
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Q5
(a) A recombinant vector with a gene of interest inserted within the gene of α-galactosidase enzyme, is introduced into a bacterium. Explain the method that would help in selection of recombinant colonies from non-recombinant ones. (b) Why is this method of selection referred to as "insertional inactivation"?