In given pedigree, unaffected parents have affected offspring which means that either one or both parents are a carrier.
It also shows recessive nature of the trait; if the trait was dominant it would have been expressed by heterozygous individuals as well. But heterozygous parents are serving as carrier only.
In offspring, both sons and daughters are not equally; sons are affected. This means disease is not autosomal; if it was autosomal, both sons and daughters of carrier parents would be equally affected.
The affected son of first offspring generation and normal female have all normal children.
This means the trait is X linked recessive trait and affected father has passed the affected X chromosome to daughter.
The daughter is carrier only because mother is normal. Likewise, normal females of first offspring generation and normal males have affected son showing that mother normal female is serving as a carrier. Trait is X-linked recessive.
Therefore, the correct answer is option D.
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