The Feulgen reaction is a technique developed by Feulgen and Rossenbeck and it is the standard technique for demonstrating deoxyribose. Mild acid hydrolysis (1M HCL, at 60oC) is used to break the purine-deoxyribose bond. The resulting aldehydes are then demonstrated by the use of Schiff's reagent. Schiff's reagent is formed by the reaction of sulfur dioxide with the dye basic fuchsin to produce a colorless compound known as leuco-basic fuchsin which reacts with exposed aldehydes and DNA is stained red-purple color. RNA does not demonstrate this reaction.
Thus, the correct answer is option B.
Was this answer helpful?
2
Similar Questions
Q1
The stain used by Feulgen to stain DNA is
View Solution
Q2
Feulgen reaction is used to stain
View Solution
Q3
Feulgen staining is a special technique for the identification of
View Solution
Q4
Question 14
The stain used to visualise mitochondria is
(a) fast green (b) safranin (c) acetocarmine (d) janus green