Unlike bacteria and other microorganisms which can be grown in artificial media, viruses cannot be grown on artificial media but must be grown in living cells. Viruses cannot be grown on an ordinary culture medium because they are not actually living organisms. Viruses can be cultivated within suitable hosts, such as a living cell. To study bacteriophages for example bacteria are grown in a suitable growth medium, then bacteriophages are added. Animal and plant viruses are cultivated in cell cultures. TMV is a virus so it can not be grown in culture medium. Escherichia coli, yeast and Aspergillus can be cultured in the medium. So the correct answer is 'TMV'.