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Question

Which of the following dye is used for staining mitochondria?
  1. Janus Green
  2. Saffranin
  3. Azure B
  4. Crystal violet

A
Janus Green
B
Azure B
C
Crystal violet
D
Saffranin
Solution
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Janus Green B is a basic dye and vital stain used in histology. It is also used to stain mitochondria supravitally. The indicator Janus Green B changes colour according to the amount of oxygen present.
If oxygen is present - Indicator is oxidised to blue colour.
If oxygen is absent - Indicator is reduced to pink colour.
Saffranin or counter stain and crystal violets are commonly used to stain bacterial cells.
Azure B is generally used to stain blood smear.

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