Which of the following restrictions enzymes produces blunt ends?
Eco RV
Hind III
Sal I
Xho I
A
Xho I
B
Sal I
C
Eco RV
D
Hind III
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Solution
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Restriction enzymes are the special enzymes which can recognise sites in the DNA sequences and make a cut at these sites. Restriction enzymes cut the DNA in middle leaving blunt ends on the DNA or staggered cuts are made that leave sticky ends with 5’or 3’ overhangs. These sticky overhangs can reanneal spontaneously by complementary base pairing. The restriction site of Eco RV is GAT_ATC and it produces blunt ends. The adaptor molecules are used to create sticky ends at the fragments which are cleaved with the help of this enzyme.
Thus, the correct answer is option D.
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Which of the following restrictions enzymes produces blunt ends?
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Which of the following restriction enzymes produce blunt ends in DNA?
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Assertion: Sticky ends forms hydrogen bonds with their complementary cut counterparts.
Reason: In recombinant DNA technology, sticky end producing restriction enzymes are preferred over blunt ends producing restriction enzymes.