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Regulation of Gene Expression

Molecular Basis of Inheritance
Biology
Class 12

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Regulation of Gene Expression

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E. coli cells with a mutated Z gene of the lac operon cannot grow in medium containing only lactose as the source of carbon because

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Lactose was added in the growth medium of E.coli. Lactose addition had induced the lac operon but it shut down after some time. What could be the possible reason?

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Explain how structural genes of lac operon start transcription on addition of lactose.

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Read the passage and answer the following question.

Shigella is a bacterium that is very closely related to E. coli but is incapable of fermenting lactose (lactose negative). A gene for resistance to the antibiotic ampicillin (amR) was inserted in between two of the structural genes of the lac operon and then inserted into Shigella.

If Shigella is normally lactose negative, from where did the lac operon originate?

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When does the lac-operon in E.coli become "switched on" 

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In a typical differentiated cell, which functional category of genes would most likely be continuously expressed?

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Choose the correct answer from the alternatives given.
The lac operon is transcribed when

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Read the passage and answer the following question.

Shigella is a bacterium that is very closely related to E. coli but is incapable of fermenting lactose (lactose negative). A gene for resistance to the antibiotic ampicillin (amR) was inserted in between two of the structural genes of the lac operon and then inserted into Shigella.

The effect of this insertion would be

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In lac operon genes A, i, Y and Z code for

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The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because

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